Danny Robinson’s side approach the fixture with confidence after a recent 5-1 thrashing of Barnstaple Town saw the Blues fly to the summit of the Southern League with two games in hand.
Enfield, meanwhile, head into Saturday’s game with renewed energy after their matches against Worthing and Bognor Regis Town were postponed in recent weeks.
The London-based club sit fourth in the Isthmian division and have only tasted defeat on one occasion in league fixtures so far this term.
Thatcham’s last outings in the competition resulted in convincing wins over Swindon Supermarine and Barnstaple Town whilst Enfield overcame Bishop’s Shortford 3-2 in the last round.